The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 1987

Filed:

Jul. 01, 1983
Applicant:
Inventors:

Alfred Puhler, Bielefeld, DE;

Reinhard Simon, Bielefeld, DE;

Ursula Priefer, Bielefeld, DE;

Assignee:

Lubrizol Genetics, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C07N / ;
U.S. Cl.
CPC ...
4351723 ; 435253 ; 435317 ; 935 26 ; 935 29 ; 935 23 ; 935 72 ; 536 27 ;
Abstract

Vectors capable of replication in a broad host range of gram-negative bacteria are provided. The vectors are composite plasmids comprising a segment derived from an E. coli vector plasmid and a segment derived from a broad host range plasmid. The broad host range plasmid segment includes Mob-functions and DNA coding for replication functions with regions non-essential for broad host range replication or mobilizablity having been deleted. Bacterial strains containing the above plasmids as well as methods of cloning DNA employing such plasmids are also provided.


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