The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 23, 1987

Filed:

Mar. 18, 1985
Applicant:
Inventors:

Kunio Matsumoto, Shizuoka, JP;

Yoshitaka Kagimoto, Shizuoka, JP;

Tsutomu Hirata, Shizuoka, JP;

Susumu Watanabe, Shizuoka, JP;

Akira Ohtsuka, Kanagawa, JP;

Kiyoharu Takahashi, Saitama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 24 ; 435-4 ; 435 23 ; 435 25 ; 435817 ;
Abstract

A method for assay of enzyme activity which comprises treating an amide compound of the formula ##STR1## with peptidase; treating thus-liberated amine of the formula ##STR2## with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula ##STR3## and quantitatively measuring the detectable changes. Also disclosed is an assay method which comprises treating an amide compound of the formula ##STR4## or a salt thereof, with peptidase to liberate an aniline derivative of the formula ##STR5## oxidizing said aniline derivative; and quantitatively measuring detectable change.


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