The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 26, 1985
Filed:
Feb. 28, 1983
Applicant:
Inventors:
Chander P Bahl, El Cerrito, CA (US);
Jack Nunberg, Oakland, CA (US);
Assignee:
Cetus Corporation, Emeryville, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435 91 ; 435 68 ; 435 70 ; 4351723 ; 435317 ; 536 27 ; 935 16 ; 935 17 ; 935 18 ;
Abstract
A method is described for constructing double-stranded DNA using messenger RNA templates. DNA strands complementary to the templates are formed using a non-specific 3' hydroxy terminus primer. After separation, the DNA single strands are disabled from self priming by blocking the free 3' hydroxy. Primer segments are then provided complementary to a portion of the amino terminus-coding end of the desired single strands. These primer segments are then extended with a suitable polymerase to complete the complementary strands.