The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 30, 1985
Filed:
Mar. 03, 1983
Stephen J Brewer, High Wycombe, GB;
Helmut M Sassenfeld, Benson, GB;
G. D. Searle & Co., Skokie, IL (US);
Abstract
A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminants by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.