The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 1985

Filed:

May. 20, 1981
Applicant:
Inventors:

Hermann G Bujard, Heidelberg, DE;

Annie C Chang, Palo Alto, CA (US);

Stanley N Cohen, Portola Valley, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12P / ; C12P / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435 29 ; 435 68 ; 4351723 ; 435 91 ; 536 27 ; 935-6 ; 935 36 ; 935 37 ; 935 41 ; 935 76 ;
Abstract

Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact promoters. These promoters are inserted into vectors separated from a balanced terminator by a gene of interest and the terminator is desirably followed by a marker allowing for selection of transformants. High efficiencies in transcription of DNA can be achieved with the highly active T5 promoters. The promoters and terminators are used in hybrid DNA for efficient expression of structural genes and transcription to provide RNA sequences.


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