The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 31, 1984
Filed:
May. 18, 1983
Wolfgang H Hanisch, Oakland, CA (US);
Peter M Fernandes, Lafayette, CA (US);
Cetus Corporation, Emeryville, CA (US);
Abstract
An improved process for recovering and purifying .beta.-HIFN from transformed bacterial comprising concentrating the bacteria; disrupting the cell wall and solubilizing the .beta.-HIFN into an aqueous medium with an appropriate solubilizing agent; extracting the .beta.-HIFN from the aqueous medium with 2-butanol, 2-methyl-2-butanol or mixtures thereof; precipitating and isolating the .beta.-HIFN from the alcohol phase; purifying the .beta.-HIFN by chromatography and diafiltering the .beta.-HIFN against distilled water or aqueous solutions of ethanol or glycerol at a pH of about 12; and therapeutic formulations and compositions of .beta.-HIFN within SDS levels therein reduced to less than 10 p.p.m.