The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 22, 1984
Filed:
Mar. 01, 1982
Applicant:
Inventors:
Michael W Konrad, Alameda, CA (US);
Leo S Lin, Fremont, CA (US);
Assignee:
Cetus Corporation, Emeryville, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07G / ; C12P / ; C12P / ; A61K / ;
U.S. Cl.
CPC ...
2601 / ; 2601 / ; 435 68 ; 435 70 ; 435811 ; 424 85 ;
Abstract
A process for recovering IFN-.beta. from transformed bacteria comprising: disrupting the cell membranes of the bacteria; solubilizing the IFN-.beta. from the disruptate into an aqueous medium with a solubilizing agent such as sodium dodecyl sulfate; extracting the IFN-.beta. from the aqueous medium with 2-butanol, 2-methyl-butanol, or mixtures thereof under conditions that maintain phase separation between the aqueous meduim and the extractant; and isolating the IFN-.beta. from the extractant such as by precipitating the IFN-.beta. from an aqeous buffer mixture of the extractant by lowering the pH thereof.