The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 1983
Filed:
Mar. 13, 1980
Applicant:
Inventors:
Francis X Cole, Stow, MA (US);
Deborah M Van Voorhis, Watertown, MA (US);
Assignee:
Millipore Corporation, Bedford, MA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435-7 ; 435 34 ; 435177 ; 435179 ; 435180 ; 436528 ; 436530 ; 436809 ; 436811 ;
Abstract
An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum. Antibody that becomes bound to the immobilized antigen is detected with an antibody- enzyme conjugate. Immobilizing the immunochemically netural protein on the membrane in a second stage immobilization step minimizes nonspecific binding during the immunoassay.