The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 21, 1982

Filed:

May. 15, 1980
Applicant:
Inventor:

Itaru Yamamoto, Okayama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435-7 ; 435188 ; 435810 ; 2323 / ; 424 12 ;
Abstract

A method of quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a specific quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the acyl-cyclic nucleotide and an enzyme through a dicarboxylic acid to undergo competitive reaction with a specific quantity of an antibody with respect to a corresponding cyclic nucleotide; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and determining the quantity of the cyclic nucleotide by measuring the enzyme activity of either of the enzyme-labeled cyclic nucleotides. For this analysis, a reagent comprising an enzyme-labeled cyclic nucleotide is used.


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