The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 31, 1982

Filed:

Jan. 12, 1981
Applicant:
Inventors:

Richard A Johnson, Clinton, IA (US);

Norman E Lloyd, Clinton, IA (US);

Assignee:

Nabisco Brands, Inc., New York, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435179 ; 435180 ; 435201 ; 435205 ; 435234 ; 435815 ;
Abstract

Enzyme purification is carried out by contacting an impure liquid enzyme preparation containing enzyme and soluble impurities with an ion exchange material in a column to adsorb both the enzyme and impurities by the ion exchange material, adding an additional amount of the impure liquid enzyme preparation whereby the soluble impurities therein are preferentially adsorbed by the ion exchange material and the adsorbed enzyme is displaced from the ion exchange material to produce a purified liquid enzyme preparation containing higher enzyme activity than before purification. The purified enzyme is more highly adsorbed by ion exchange material when immobilizing the enzyme.


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