The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 27, 1982
Filed:
Nov. 19, 1980
Richard G Wyatt, Potomac, MD (US);
Walter D James, Beltsville, MD (US);
Edward H Bohl, Wooster, OH (US);
Kenneth W Theil, Wooster, OH (US);
Linda J Saif, Wooster, OH (US);
Anthony R Kalica, Rockville, MD (US);
Harry B Greenberg, Washington, DC (US);
Albert Z Kapikian, Rockville, MD (US);
Robert M Chanock, Bethesda, MD (US);
Abstract
This invention relates to a precursor or intermediate for a rotavirus vaccine to protect infants and young children against diarrhea caused by rotavirus. The invention is the efficient propagation of a strain of human rotavirus type 2 prepared by a method of cultivation by multiple passages in vivo in gnotobiotic piglets and by multiple passages in vitro subsequently in AGMK (African green monkey kidney) cell cultures. Additionally, rotavirus is treated with a biologically active amount of trypsin prior to growth in AGMK cells, and the inoculated cell cultures are centrifuged at a low centrifugal force before incubation.