The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 21, 1981
Filed:
Jun. 12, 1979
Khemchand M Shahani, Lincoln, NE (US);
Frederick W Wagner, Walton, NE (US);
Arun Kilara, State College, PA (US);
The Board of Regents of the University of Nebraska, Lincoln, NE (US);
Abstract
To permit regeneration of an insoluble support for immobilized proteins, the protein is connected to the support by a spacer arm containing a diazo linkage and when the protein is denatured, the spacer arm is broken by reducing the diazo linkage to remove the denatured protein. New native protein of the same or of a different type is joined to the arm to permit reuse of the support. A preferred support containing a spacer arm is p-(N-acetyl-L-tyrosine azo)benzamidoethyl agarose. A protein is coupled to the carboxyl group of N-acetyl-L-tyrosine and when the protein is spent, the azo bond is reduced to remove the protein and leave p-aminobenzamido agarose which is diazotized and recoupled to N-acetyl-L-tyrosine which is then coupled to fresh protein.