The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 23, 1980
Filed:
Aug. 14, 1978
Mathew M Madappally, Cooper City, FL (US);
Giovanni Bucolo, Cupertino, CA (US);
Coulter Electronics, Inc., Hialeah, FL (US);
Abstract
An improvement in a method for determining glutamate oxalacetate transaminase or glutamate pyruvate transaminase present in a biological fluid, in which method L-glutamate is produced from alpha-ketoglutarate by transamination in the presence of the transaminase in the fluid, and the L-glutamate is oxidatively deaminated in the presence of glutamate dehydrogenase with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the transaminase in the fluid, such fluid also containing an endogenous substance which is oxidized in the presence of glutamate dehydrogenase with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide, thereby interfering with the determination. The improvement for obviating the interference caused by the endogenous substance includes oxidizing the endogenous substance in the presence of glutamate dehydrogenase with the simultaneous reduction of beta-nicotinamide adenine dinucleotide, oxidizing the reduced beta-nicotinamide adenine dinucleotide in the presence of lactate dehydrogenase with the simultaneous reduction of pyruvate to lactate, thereafter inhibiting the lactate dehydrogenase activity, and thereafter producing a quantity of L-glutamate from alpha-ketoglutarate by the aforesaid transamination for oxidative deamination thereof and simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the transaminase in the fluid. A reagent combination is employed for the determination.