The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 01, 1980

Filed:

Oct. 13, 1977
Applicant:
Inventors:

Wolfgang Gruber, Tutzing-Unterzeismering, DE;

Gunter Lang, Tutzing, DE;

Michael Nelboeck-Hochstetter, Tutzing, DE;

Peter Roeschlau, Seeshaupt, DE;

Hans Seidel, Tutzing, DE;

Detlef von Hoerschelmann, Wielenbach, DE;

Assignee:

Boehringer Mannheim GmbH, Mannheim-Waldhof, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
435 11 ;
Abstract

In a process for the determination of total or bound cholesterol by liberating any bound cholesterol using cholesterol esterase and simultaneously or subsequently determining the liberated cholesterol and any initially free cholesterol with a cholesterol reacting enzyme, the improvement comprising using as the cholesterol reacting enzyme an NAD or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic microorganism, e.g., Eubacterium sp. ATCC 21408, or from mammalian tissue, e.g., liver.


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