The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 02, 1978
Filed:
Jan. 12, 1977
Giovanni Bucolo, Hialeah, FL (US);
Mathew M Madappally, Cooper City, FL (US);
Gerald E Jaffe, Miami, FL (US);
Coulter Electronics, Inc., Hialeah, FL (US);
Abstract
A colorimetric method for the determination of gamma-glutamyl transpeptidase enzyme activity in a biological fluid comprises catalytically reacting the enzyme in the fluid with a substrate of a water-soluble salt of a compound selected from the group consisting of L-gamma-glutamyl-3-sulfoanilide and L-gamma-glutamyl-4-sulfoanilide in aqueous solution to produce the corresponding aniline sulfonic acid salt as a cleavage product, diazotizing the cleavage product, and coupling the diazotized product with a coupling compound to produce a dye having a color intensity proportional to the enzyme activity.