Single-cell-based electrochemical sensor based on functionalized nano-probe and application thereof

Abstract

The disclosure provides a single-cell-based electrochemical sensor based on a functionalized nano-probe and an application thereof, and belongs to the technical fields of electrochemical sensors and toxin detection. The single-cell-based electrochemical sensor of the disclosure combines a nano-probe and an electrochemical cell-based sensor, conducts functional modification on the nano-probe using Prussian blue, and conducts current signal analysis on a single cell by a micro-operating platform. The disclosure constructs a reliable, easy to operate and highly repeatable single-cell-based electrochemical detection platform, and the current value is determined by electrochemical chronoamperometry to determine damage of a single cell stimulated by toxins, thereby quickly and effectively evaluating the cytotoxicity of fungal toxins, and further enabling application of the fungal toxin toxicity in real-time monitoring and nano-environmental detection in living cells.