The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2026

Filed:

Jul. 05, 2016
Applicant:

Hitachi, Ltd., Tokyo, JP;

Inventors:

Junko Tanaka, Tokyo, JP;

Yoshinobu Kohara, Tokyo, JP;

Kunio Harada, Tokyo, JP;

Yu Ishige, Tokyo, JP;

Assignee:

Hitachi, Ltd., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); B01J 19/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/68 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01); G16B 25/20 (2019.01); G16B 99/00 (2019.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01J 19/0046 (2013.01); C12M 1/00 (2013.01); C12Q 1/68 (2013.01); C12Q 1/686 (2013.01); G16B 25/20 (2019.02); G16B 99/00 (2019.02); B01L 3/502784 (2013.01); B01L 7/52 (2013.01); C12Q 2561/12 (2013.01); C12Q 2563/107 (2013.01); C12Q 2563/173 (2013.01);
Abstract

The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.


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