The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 12, 2026

Filed:

Nov. 18, 2021
Applicant:

Akoya Biosciences, Inc., Menlo Park, CA (US);

Inventors:

Yi Zheng, Dover, MA (US);

Julia Kennedy-Darling, Redwood City, CA (US);

Peter J. Miller, Cambridge, MA (US);

Assignee:

Akoya Biosciences, Inc., Menlo Park, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); B82Y 5/00 (2011.01); B82Y 30/00 (2011.01); B82Y 40/00 (2011.01); C07F 5/02 (2006.01); C09B 57/00 (2006.01); C09K 11/06 (2006.01); G01N 1/30 (2006.01); G01N 15/10 (2024.01); G01N 15/1429 (2024.01); G01N 27/626 (2021.01); G01N 31/22 (2006.01); G01N 33/483 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/582 (2013.01); G01N 1/30 (2013.01); G01N 33/54346 (2013.01); G01N 33/6854 (2013.01); B82Y 5/00 (2013.01); B82Y 30/00 (2013.01); B82Y 40/00 (2013.01);
Abstract

Methods include (a) contacting a biological sample with a composition featuring a plurality of different types of probes, where each type of probe of the plurality of different types of probes includes a detection moiety that selectively binds to a different type of protein target in the sample, a nanobody bound to the detection moiety, and an oligonucleotide linked to the nanobody and featuring an oligonucleotide sequence, where the oligonucleotide sequence of each type of probe is different from the oligonucleotide sequences of each of the other types of probes of the plurality of probes; and (b) contacting the sample with a set of one or more different types of optical labels, where each different type of optical label of the set of optical labels includes an oligonucleotide that selectively hybridizes to only one type of probe.


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