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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12497581 B1

PDFFull Text
Date of Patent:
Dec. 16, 2025

Filed:

May. 16, 2024

High-throughput microfluidic chip having parallelized constrictions for perturbing cell membranes

Applicant:

Stemcell Technologies Canada Inc., Vancouver, CA;

Inventors:

Maisam Dadgar, Cambridge, MA (US);

Jacquelyn L. Sikora Hanson, Watertown, MA (US);

Armon R. Sharei, Somerville, MA (US);

Assignee:

STEMCELL TECHNOLOGIES CANADA INC., Vancouver, CA;

Attorneys:

SMART & BIGGAR LP

Micheline Gravelle

Primary Examiner:

Jennifer Wecker

Assistant Examiner:

Oyeleye Alexander Alabi

Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01F 33/302 (2022.01); B01F 33/3033 (2022.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01); B23D 63/00 (2006.01); B65G 47/80 (2006.01); B82Y 20/00 (2011.01); B82Y 30/00 (2011.01); B82Y 40/00 (2011.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 3/06 (2006.01); C12N 1/14 (2006.01); C12N 1/20 (2006.01); C12Q 1/02 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C23C 2/00 (2006.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1433 (2024.01); G01N 21/29 (2006.01); G01N 21/45 (2006.01); G01N 21/65 (2006.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01); G01N 33/58 (2006.01); G02B 6/42 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); B01L 3/502707 (2013.01); C12M 33/12 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0819 (2013.01);
Abstract

A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

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