The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 04, 2025
Filed:
Dec. 06, 2019
Applicant:
William Marsh Rice University, Houston, TX (US);
Inventors:
Jeffrey J. Tabor, Houston, TX (US);
Sebastian M. Castillo-Hair, Houston, TX (US);
Assignee:
WILLIAM MARSH RICE UNIVERSITY, Houston, TX (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/75 (2006.01); C12N 1/20 (2006.01); C12N 15/63 (2006.01); C12R 1/125 (2006.01);
U.S. Cl.
CPC ...
C12N 15/75 (2013.01); C12N 1/20 (2013.01); C12N 15/635 (2013.01); C12R 2001/125 (2021.05);
Abstract
An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into thegenome. In the absence of IPTG, Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.