The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 16, 2025

Filed:

Jan. 17, 2025
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Yao Nie, Wuxi, CN;

Yan Xu, Wuxi, CN;

Songyin Zhao, Wuxi, CN;

Jiaojiao Guan, Wuxi, CN;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 1/20 (2006.01); C12N 15/70 (2006.01); C12P 17/12 (2006.01); C12R 1/19 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0071 (2013.01); C12N 1/20 (2013.01); C12N 15/70 (2013.01); C12P 17/12 (2013.01); C12R 2001/19 (2021.05); C12Y 114/11028 (2013.01);
Abstract

By mutating arginine at position 97 and asparagine at position 43 of a wild type L-proline-3-hydroxylase, a mutated L-proline-3-hydroxylase is obtained. The mutated L-proline-3-hydroxylase comprises a variant of the wild-type L-proline-3-hydroxylase having SEQ ID NO:1, which comprises a substitution of the arginine corresponding to position 97 (R97) of SEQ ID NO:1 and a substitution of the asparagine corresponding to position 43 (N43) of SEQ ID NO:1; wherein the amino acid substituted into the mutated L-proline-3-hydroxylase at position R97 is methionine, and wherein the amino acid substituted into the mutated L-proline-3-hydroxylase at position N43 is selected from the group consisting of threonine (T), alanine (A), valine (V), serine(S), aspartic acid (D), and lysine (K).


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