The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2025
Filed:
Aug. 24, 2020
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
G. Brett Robb, Somerville, MA (US);
Siu-Hong Chan, Georgetown, MA (US);
Bijoyita Roy, Medford, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 19/20 (2006.01); C07H 21/04 (2006.01); C12P 19/34 (2006.01); C12Q 1/6816 (2018.01);
U.S. Cl.
CPC ...
C07H 19/20 (2013.01); C07H 21/04 (2013.01); C12P 19/34 (2013.01); C12Q 1/6816 (2013.01);
Abstract
Provided herein is a method for efficiently capping RNA in vitro. In some embodiments the capping reaction may be done at high temperature using Vaccinia capping enzyme or a variant thereof. In other embodiments, the capping reactions may comprise a capping enzyme from a large virus of amoeba, e.g., Faustovirus, mimivirus or moumouvirus, or a variant thereof. Compositions and kits for practicing the method are also provided.