The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2025
Filed:
Jan. 08, 2025
Jiangsu Academy of Agricultural Sciences, Nanjing, CN;
Snu R&db Foundation, Seoul, KR;
Guotai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, Taizhou, CN;
Xing Xie, Nanjing, CN;
Zhixin Feng, Nanjing, CN;
Daesub Song, Seoul, KR;
Rong Chen, Nanjing, CN;
Fei Hao, Nanjing, CN;
Lulu Xu, Nanjing, CN;
Minjoo Yeom, Seoul, KR;
Jongwoo Lim, Seoul, KR;
Lei Zhang, Nanjing, CN;
Yuan Gan, Nanjing, CN;
Long Zhao, Nanjing, CN;
Wenliang Li, Nanjing, CN;
Qiyan Xiong, Nanjing, CN;
Yongjie Liu, Nanjing, CN;
Beibei Liu, Nanjing, CN;
Yanfei Yu, Nanjing, CN;
Yun Bai, Nanjing, CN;
Guoqing Shao, Nanjing, CN;
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, Nanjing, CN;
SNU R&DB FOUNDATION, Seoul, KR;
GUOTAI (TAIZHOU) CENTER OF TECHNOLOGY INNOVATION FOR VETERINARY BIOLOGICALS, TAIZHOU, Taizhou, CN;
Abstract
A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 10TCID/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.