The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 22, 2025

Filed:

Nov. 01, 2023
Applicants:

Jiangnan University, Jiangsu, CN;

Inner Mongolia Mengniu Dairy (Group) Company Limited, Inner Mongolia, CN;

Inventors:

Long Liu, Wuxi, CN;

Jian Chen, Wuxi, CN;

Xueqin Lv, Wuxi, CN;

Wenyang Wu, Wuxi, CN;

Guocheng Du, Wuxi, CN;

Jianghua Li, Wuxi, CN;

Guolin Zhou, Wuxi, CN;

Yanfeng Liu, Wuxi, CN;

Chenyang Zhang, Wuxi, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C12N 9/10 (2006.01); C12N 9/20 (2006.01); C12P 7/6409 (2022.01);
U.S. Cl.
CPC ...
C12P 7/6409 (2013.01); C12N 9/1029 (2013.01); C12N 9/20 (2013.01); C12N 15/81 (2013.01); C12Y 203/01051 (2013.01); C12Y 301/01003 (2013.01); C12N 2800/102 (2013.01);
Abstract

The invention provides astrain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase intoand knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced byis increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized byfor the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.


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