The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 15, 2025
Filed:
Sep. 24, 2021
Applicant:
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Inventors:
Tomoyuki Igawa, Shizuoka, JP;
Hitoshi Katada, Shizuoka, JP;
Yuji Hori, Shizuoka, JP;
Shojiro Kadono, Kanagawa, JP;
Assignee:
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/46 (2006.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C07K 16/464 (2013.01); C07K 16/00 (2013.01); C07K 16/2866 (2013.01); C07K 16/468 (2013.01); G01N 33/15 (2013.01); G01N 33/50 (2013.01); G01N 33/53 (2013.01); C07K 2317/31 (2013.01); C07K 2317/526 (2013.01); C07K 2317/53 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/624 (2013.01);
Abstract
It is intended to provide a method for efficiently and stably producing a heteromultimer by incubating homo variants of plural types of heavy chain constant region-containing polypeptides differing in antigen-binding activity under a reducing condition that reorganize the inter-polypeptide disulfide bond between cysteine residues outside of core hinge regions. A feature of the production method of the present invention is that amino acid residues in the core hinge regions do not form any disulfide bond.