The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 24, 2025

Filed:

Jun. 23, 2020
Applicant:

Toyota Jidosha Kabushiki Kaisha, Toyota, JP;

Inventors:

Toru Onishi, Toyota, JP;

Nobuki Tada, Nisshin, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 15/00 (2006.01); C12N 15/65 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 15/905 (2013.01); C12N 15/00 (2013.01); C12N 15/65 (2013.01); C12N 15/81 (2013.01); C12N 2800/80 (2013.01);
Abstract

A stable transformant, in which a gene of interest is incorporated into the genome, is simply and efficiently produced. A method for producing a transformant, comprising a step of introducing into a host, linear genome-introduced nucleic acid fragment(s) comprising a gene of interest and a helper plasmid for transformation having a pair of homologous recombination sequences for incorporation of the linear genome-introduced nucleic acid fragment(s), and then selecting a transformant, in which the gene of interest is incorporated into the predetermined position in the host genome and the gene of interest is expressed therein.


Find Patent Forward Citations

Onishi, Toru. (2025). Helper plasmid for transformation, method for producing transformant using the same, and transformation method (U.S. Patent No. 12338454). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12338454

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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