The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 10, 2025

Filed:

Jun. 05, 2019
Applicant:

Ucb Biopharma Srl, Brussels, BE;

Inventors:

Ralph Adams, Slough, GB;

James Heads, Slough, GB;

Sebastian Kelm, Slough, GB;

Alastair David Griffiths Lawson, Slough, GB;

Assignee:

UCB BIOPHARMA SRL, Brussels, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G16B 15/20 (2019.01); C07K 16/00 (2006.01); G16B 15/00 (2019.01); G16B 30/00 (2019.01); G16B 40/00 (2019.01); G16C 20/50 (2019.01); G16B 5/00 (2019.01);
U.S. Cl.
CPC ...
G16B 15/20 (2019.02); C07K 16/00 (2013.01); G16B 15/00 (2019.02); G16B 30/00 (2019.02); G16B 40/00 (2019.02); G16C 20/50 (2019.02); C07K 2317/52 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01); G16B 5/00 (2019.02);
Abstract

The present invention provides a method for generation of multi-domain proteins, more particular antibodies, with an improved native state colloidal stability. The present disclosure provides a method of producing an IgG1 or IgG4 antibody with an improved colloidal stability comprising, in embodiments: calculating for each domain of said IgG1 or IgG4 antibody the total net charge at a given pH; introducing one or more modifications to the amino acid residues of the constant region of said IgG1 or IgG4 antibody to minimize the charge sign difference between the domains, wherein said one or more modification is, or each are, a substitution of a charged amino acid by a polar (non-charged) amino acid; and producing the modified multi-domain protein with improved colloidal stability at the given pH.


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