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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12270028 B1

PDFFull Text
Date of Patent:
Apr. 08, 2025

Filed:

Apr. 13, 2022

Methods for seamless nucleic acid assembly

Applicant:

Twist Bioscience Corporation, South San Francisco, CA (US);

Inventors:

Rebecca Nugent, San Francisco, CA (US);

Siyuan Chen, San Mateo, CA (US);

Elian Lee, Union City, CA (US);

Nathan Raynard, San Francisco, CA (US);

Assignee:

Twist Bioscience Corporation, South San Francisco, CA (US);

Attorney:

Bookoff McAndrews, PLLC

Primary Examiner:

Narayan K Bhat

Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 9/00 (2006.01); C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1096 (2013.01); C12N 9/1252 (2013.01); C12N 9/93 (2013.01); C12N 15/1031 (2013.01); C12N 15/1093 (2013.01); C12P 19/34 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01); C12Q 2531/101 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/11002 (2013.01);
Abstract

Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

Find Patent Forward Citations

Nugent, Rebecca. (2025). Methods for seamless nucleic acid assembly (U.S. Patent No. 12270028). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12270028

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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