The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 04, 2025

Filed:

May. 08, 2018
Applicant:

Bristol-myers Squibb Company, Princeton, NJ (US);

Inventors:

Cheng Du, West Roxbury, MA (US);

Ameya Umesh Borwankar, San Francisco, CA (US);

Joon Chong Yee, Acton, MA (US);

Zhijun Tan, Acton, MA (US);

Assignee:

BRISTOL-MYERS SQUIBB COMPANY, Princeton, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 16/00 (2013.01); C07K 2317/14 (2013.01);
Abstract

The invention is a method of preventing the generation of pink color during antibody manufacturing by either preventing the reduction of antibodies during manufacturing and harvest or inhibiting the conversion of cyanocobalamin (CN-Cbl) to hydroxocobalamin (HO-Cbl) during manufacturing and harvest. Replacement of white light in the cell culture media preparation and storage areas with red light inhibits the conversion of CN-Cbl to HO-Cbl. Addition of peroxides to the clarified bulk at harvest inhibits reduction of the antibody disulfide bonds.


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