The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 2025

Filed:

Apr. 20, 2020
Applicant:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Inventors:

Geoffrey Barrall, San Diego, CA (US);

Takeshi Harada, San Jose, CA (US);

Jason Komadina, Fremont, CA (US);

Pirooz Parvarandeh, Los Altos Hills, CA (US);

Charlotte Yang, San Jose, CA (US);

Assignee:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
B01L 3/502 (2013.01); C12Q 1/6869 (2013.01); B01L 2200/12 (2013.01); B01L 2200/143 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0896 (2013.01); B01L 2400/06 (2013.01);
Abstract

Techniques for replacing nanopores within a nanopore based sequencing chip are provided. A first electrolyte solution is added to the external reservoir of the sequencing chip, introducing an osmotic imbalance between the reservoir and the well chamber located on the opposite side of a lipid bilayer membrane. The osmotic imbalance causes the membrane to change shape, and a nanopore within the membrane to be ejected. A second electrolyte solution is then added to the external reservoir to provide replacement nanopores and to restore the membrane shape. The replacement nanopores can be inserted into the membrane, effectively replacing the initial pore without causing the destruction of the membrane.


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