The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2025
Filed:
Dec. 21, 2018
Applicant:
The Governing Council of the University of Toronto, Toronto, CA;
Inventors:
Assignee:
The Governing Council of the University of Toronto, Toronto, CA;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/02 (2006.01); C12P 19/14 (2006.01); C12Q 1/54 (2006.01);
U.S. Cl.
CPC ...
C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12Q 1/54 (2013.01); C12Y 101/01047 (2013.01); C12Y 301/03009 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/0102 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01023 (2013.01); C12Y 302/01026 (2013.01); C12Y 302/01028 (2013.01);
Abstract
Described are methods for generating a reporter molecule in response to a target analyte in a cell-free system. A synthetic biological circuit is used to modify the level of the reporter molecule in response to the presence of the target analyte. The reporter molecule may be glucose or another molecule readily detected using a device such as glucose monitor or other portable sensor. Also provided are kits comprising a cell-free system with a synthetic biological circuit that generates or consumes a reporter molecule in response to a target analyte.