The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2025
Filed:
Jul. 19, 2018
I'rom Group Co., Ltd., Tokyo, JP;
Kohji Kusano, Tokyo, JP;
Takayuki Kitogo, Tokyo, JP;
Makoto Inoue, Tokyo, JP;
Tsugumine Shu, Tokyo, JP;
Toyotaka Mori, Tokyo, JP;
I'ROM GROUP CO., LTD., Tokyo, JP;
Abstract
The present invention provides donor polynucleotides formed by linking the two ends of a genomic fragment containing a cleavable site by a polynucleotide carrying a positive selection marker gene and a negative selection marker gene. Use of the donor polynucleotide makes it possible to modify only a target gene with avoiding the possibility of introducing mutations to sequences, called 'off-target', which are other than the target sequence, by introducing cleavage in a homologous site of the donor polynucleotide without introducing cleavage in a target gene locus.