The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 21, 2025

Filed:

Jan. 25, 2019
Applicant:

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, CN;

Inventors:

Yuanxia Sun, Tianjin, CN;

Jiangang Yang, Tianjin, CN;

Yunjie Li, Tianjin, CN;

Yueming Zhu, Tianjin, CN;

Chun You, Tianjin, CN;

Yanhe Ma, Tianjin, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12N 9/04 (2006.01); C12N 9/16 (2006.01); C12N 9/90 (2006.01); C12N 9/92 (2006.01); C12P 19/02 (2006.01); C12P 19/24 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1205 (2013.01); C12N 9/0006 (2013.01); C12N 9/12 (2013.01); C12N 9/16 (2013.01); C12N 9/90 (2013.01); C12N 9/92 (2013.01); C12P 19/02 (2013.01); C12P 19/24 (2013.01); C12Y 101/01006 (2013.01); C12Y 101/01056 (2013.01); C12Y 207/01002 (2013.01); C12Y 207/01004 (2013.01); C12Y 207/01011 (2013.01); C12Y 301/03039 (2013.01); C12Y 503/01009 (2013.01);
Abstract

Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.


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