The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 14, 2025

Filed:

Jun. 23, 2021
Applicant:

Boehringer Ingelheim International Gmbh, Ingelheim am Rhein, DE;

Inventors:

Gregory Walter Hiller, Wakefield, MA (US);

Matthew Paul Gagnon, Medford, MA (US);

Jonathan Coffman, Gaithersbury, MD (US);

Assignee:

Boehringer Ingelheim International GmbH, Ingelheim am Rhein, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
H04M 1/02 (2006.01); C07K 16/00 (2006.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C12P 21/02 (2006.01); G06F 1/16 (2006.01); G09F 9/30 (2006.01);
U.S. Cl.
CPC ...
C12M 23/58 (2013.01); C07K 16/00 (2013.01); C12M 23/40 (2013.01); C12M 27/02 (2013.01); C12M 29/10 (2013.01); C12N 5/0018 (2013.01); C12N 5/0682 (2013.01); C12P 21/02 (2013.01); C07K 2317/24 (2013.01);
Abstract

Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N−1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N−1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.


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