The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 05, 2024

Filed:

Oct. 19, 2023
Applicant:

Children's Hospital Los Angeles, Los Angeles, CA (US);

Inventors:

Laura Perin, Burbank, CA (US);

Stefano Da Sacco, Glendale, CA (US);

Roger De Filippo, Glendale, CA (US);

Assignee:

Children's Hospital Los Angeles, Los Angeles, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); C12M 25/02 (2013.01); C12M 29/10 (2013.01); C12N 5/0686 (2013.01); G01N 33/5088 (2013.01);
Abstract

A glomerulus on a chip (GOAC) to recapitulate the human glomerular filtration barrier, the structure responsible for filtering the blood and preventing the loss of proteins, is provided using human podocytes and glomerular endothelial cells seeded into microfluidic chips. In long-term cultures, cells maintain their morphology, form capillary-like structures and express slit diaphragm proteins. This system recapitulates functions and structure of the glomerulus, including permselectivity. When exposed to sera from patients with anti-podocyte autoantibodies, the chips show albuminuria proportional to patients' proteinuria, phenomenon not observed with sera from healthy controls or individuals with primary podocyte defects. Also shown is its applicability for renal disease modeling and drug testing.


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