The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2024
Filed:
May. 14, 2019
Illumina, Inc., San Diego, CA (US);
Illumina Cambridge Limited, Cambridge, GB;
Xiaolin Wu, Cambridge, GB;
Randall Smith, San Diego, CA (US);
Peyton Shieh, Cambridge, MA (US);
John M. Beierle, Carlsbad, CA (US);
Wayne N. George, Cambridge, GB;
Elliot John Lawrence, Cambridge, GB;
Jie Mao, San Diego, CA (US);
Xiaohai Liu, Cambridge, GB;
Illumina, Inc., San Diego, CA (US);
Abstract
Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by NaSO. Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.