The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 2024
Filed:
Aug. 17, 2022
Henan Julong Biological Engineering Co., Ltd, Henan, CN;
Tianjin University of Science and Technology, Tianjin, CN;
Xiaoguang Fan, Tianjin, CN;
Xiaodong Liu, Henan, CN;
Jing Li, Henan, CN;
Ning Chen, Tianjin, CN;
Bochao Liu, Henan, CN;
Shuai Liu, Henan, CN;
Chaochao Sun, Henan, CN;
Yongchao Liu, Henan, CN;
Jiajia Teng, Henan, CN;
Mengtao Zhang, Henan, CN;
Yuanqing Ji, Tianjin, CN;
Yuhang Zhou, Tianjin, CN;
Qingyang Xu, Tianjin, CN;
Henan Julong Biological Engineering Co., Ltd, Ruzhou, CN;
Tianjin University of Science and Technology, Tianjin, CN;
Abstract
The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using angenetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on anW3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.