The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 20, 2024

Filed:

Feb. 13, 2018
Applicant:

Takara Bio Usa, Inc., Mountain View, CA (US);

Inventors:

Magnolia Bostick, San Mateo, CA (US);

Ishminder Mann, Milpitas, CA (US);

Andrew Alan Farmer, Los Altos, CA (US);

Sarah Taylor, Sunnyvale, CA (US);

Assignee:

Takara Bio USA, Inc., San Jose, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6853 (2018.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1086 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/686 (2013.01); C12Q 2521/107 (2013.01); C12Q 2525/191 (2013.01);
Abstract

Methods of preparing nucleic acid libraries are provided. Aspects of the methods include producing one or more libraries, including e.g., expression libraries and/or immune cell receptor repertoire libraries, from double stranded complementary DNA (cDNA) generated through a template-switching reaction involving a RNA sample. In some aspects, the methods include preparing a library from a single cell and/or a library indexed at the single cell level. Compositions and kits for use in performing the methods are also provided.


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