The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 13, 2024
Filed:
May. 10, 2018
Applicant:
Argenx Bv, Ghent, BE;
Inventors:
Christophe Blanchetot, Ghent, BE;
Erik Hofman, Ghent, BE;
Johannes De Haard, Ghent, BE;
Jacobus Cornelis Rasser, Ghent, BE;
Assignee:
argenx BV, Ghent, BE;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/18 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/18 (2013.01); C07K 16/00 (2013.01); C07K 2317/22 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01); G01N 2500/04 (2013.01);
Abstract
Methods for preparing engineered antibodies exhibiting improved pH-dependent antigen binding are disclosed. The methods are based on introduction of histidine residues at a subset of defined amino acid positions within the antibody CDRs. The set of amino acid positions selected for histidine substitution is derived from a heat-map of histidine occurrence within the CDRs of functional antibodies from a natural antibody repertoire. The methods provide a simpler and less time-consuming approach to the identification of pH-dependent antibody variants.