The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 30, 2024
Filed:
Sep. 20, 2021
Jiangnan University, Wuxi, CN;
Liming Liu, Wuxi, CN;
Guipeng Hu, Wuxi, CN;
Xiulai Chen, Wuxi, CN;
Danlei Ma, Wuxi, CN;
Jia Liu, Wuxi, CN;
Qiuling Luo, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The disclosure discloses construction and application of an engineered strain offor producing malic acid by fixing CO, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation onMG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with COand glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.