The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 18, 2024
Filed:
Jun. 11, 2019
The Broad Institute, Inc., Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
The Children's Medical Center Corporation, Boston, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Leif S. Ludwig, Cambridge, MA (US);
Caleb A. Lareau, Boston, MA (US);
Jacob C. Ulirsch, Boston, MA (US);
Aviv Regev, Cambridge, MA (US);
Vijay G. Sankaran, Boston, MA (US);
Jason Buenrostro, Cambridge, MA (US);
Christoph Muus, Cambridge, MA (US);
The Broad Institute, Inc., Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
The Children's Medical Center Corporation, Boston, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Abstract
Embodiments disclosed herein provide methods of using somatic mutations in mitochondrial genomes to retrospectively infer cell lineages in native contexts and to serve as genetic barcodes to measure clonal dynamics in complex cellular populations. Further, somatic mutations in mitochondrial DNA (mtDNA) are tracked by single cell genomic approaches for simultaneous analysis of single cell lineage and state. Applicants further show that mitochondrial mutations can be readily detected with contemporary single cell transcriptomic and epigenomic technologies to concomitantly capture gene expression profiles and chromatin accessibility, respectively.