The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 04, 2024
Filed:
Jan. 13, 2022
Integrated Dna Technologies, Inc., Coralville, IA (US);
Joseph Dobosy, Coralville, IA (US);
Scott D. Rose, Coralville, IA (US);
Jeffrey A. Manthey, North Liberty, IA (US);
Shawn D. Allen, Williamsburg, IA (US);
Steven A. Henck, Mountain View, CA (US);
Mark Behlke, Coralville, IA (US);
INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US);
Abstract
Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.