The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 2024
Filed:
Sep. 17, 2015
Global Life Sciences Solutions Usa Llc, Marlborough, MA (US);
Ryan Charles Heller, Niskayuna, NY (US);
Nichole Lea Wood, Niskayuna, NY (US);
Robert Scott Duthie, Niskayuna, NY (US);
John Richard Nelson, Niskayuna, NY (US);
Wei Gao, Niskayuna, NY (US);
Michael James Rishel, Niskayuna, NY (US);
Klaus Gustav Hentrich, Cardiff, GB;
GLOBAL LIFE SCIENCES SOLUTIONS USA LLC, Marlborough, MA (US);
Abstract
A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.