The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2024
Filed:
Nov. 12, 2020
Jiangnan University, Wuxi, CN;
Liming Liu, Wuxi, CN;
Wenxiu Tang, Wuxi, CN;
Chen Shen, Wuxi, CN;
Qiuling Luo, Wuxi, CN;
Xiulai Chen, Wuxi, CN;
Jia Liu, Wuxi, CN;
Cong Gao, Wuxi, CN;
Wei Song, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The invention provides a recombinantstrain for producing succinic acid and a construction method thereof. The by-product encoding genes in thestrain FMME-N-2 are knocked out to obtain thestrain FMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA); and the phosphoenolpyruvate carboxykinase pck derived fromand the phosphite dehydrogenase ptxD derived fromwere overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression hostFMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strainFMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.43 g/L/h, while no by-products of lactic acid and formic acid are accumulated, and the acetic acid content is 1-2 g/L.