The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 02, 2024
Filed:
Aug. 23, 2018
Applicant:
Universität Zürich, Zürich, CH;
Inventors:
Assignee:
UNIVERSITÄT ZÜRICH, Zürich, CH;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/0784 (2010.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); C12N 5/0786 (2010.01); C12Q 1/6883 (2018.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0645 (2013.01); C12N 5/0639 (2013.01); C12N 5/0656 (2013.01); C12N 5/0697 (2013.01); C12Q 1/6883 (2013.01); G01N 33/6893 (2013.01); C12N 2500/34 (2013.01); C12N 2501/727 (2013.01); C12N 2502/1323 (2013.01); C12N 2513/00 (2013.01);
Abstract
The invention provides a method for the generation of a layered cellular 3 D microtissue aggregate, comprising the steps of contacting myeloid cells with a protein kinase C agonist, yielding primed myeloid cells; incubating the primed myeloid cells in the presence of LDL in a confined volume, particularly in a hanging drop culture; yielding a 3 D culture of myeloid cells; and incubating the 3 D culture together with fibroblasts in a hanging drop in the presence of LDL, yielding the layered cellular aggregate.