The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 26, 2024

Filed:

Mar. 30, 2022
Applicant:

Cargill, Incorporated, Wayzata, MN (US);

Inventors:

Chris Miller, Andover, MN (US);

Brian Rush, Minneapolis, MN (US);

Joshua Dunn, Boston, MA (US);

Brynne Stanton, Boston, MA (US);

Assignee:

CARGILL, INCORPORATED, Wayzata, MN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/10 (2006.01); C12N 1/18 (2006.01); C12N 9/34 (2006.01); C12R 1/865 (2006.01);
U.S. Cl.
CPC ...
C12P 7/10 (2013.01); C12N 9/2428 (2013.01); C12N 1/185 (2021.05); C12P 2203/00 (2013.01); C12R 2001/865 (2021.05); C12Y 302/01003 (2013.01);
Abstract

The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.


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