The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 19, 2024

Filed:

Dec. 22, 2018
Applicant:

Enzymaster (Ningbo) Bio-engineering Co., Ltd, Ningbo, CN;

Inventors:

Haibin Chen, Ningbo, CN;

Yong Koy Bong, Ningbo, CN;

Juanjuan Wang, Ningbo, CN;

Baoqin Cai, Ningbo, CN;

Chuanyang Shang, Ningbo, CN;

Marco Bocola, Ningbo, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 13/00 (2006.01); C12N 9/10 (2006.01); C12N 11/00 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 13/001 (2013.01); C12N 9/1096 (2013.01); C12N 11/00 (2013.01); C12N 15/70 (2013.01);
Abstract

Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.


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