The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2024

Filed:

Apr. 27, 2021
Applicants:

Imperial College Innovations Limited, London, GB;

Institut Pasteur, Paris, FR;

Inventors:

Felix Rey, Paris, FR;

Giovanna Barba Spaeth, Paris, FR;

Marie-Christine Vaney, Paris, FR;

Alexander Rouvinski, Jerusalem, IL;

Gavin Screaton, London, GB;

Juthathip Mongkolsapaya, London, GB;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/10 (2006.01); A61K 39/00 (2006.01); A61K 39/12 (2006.01); A61P 31/14 (2006.01); C07K 14/005 (2006.01); C07K 14/18 (2006.01); C07K 14/705 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); A61K 39/12 (2013.01); A61P 31/14 (2018.01); C07K 14/1825 (2013.01); C07K 16/1081 (2013.01); G01N 33/56983 (2013.01); C07K 2317/21 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/565 (2013.01); C07K 2317/76 (2013.01); C12N 2770/24122 (2013.01); C12N 2770/24134 (2013.01); C12N 2770/24171 (2013.01); Y02A 50/30 (2018.01);
Abstract

A flavivirus Envelope Dimer Epitope (EDE) and isolated neutralizing antibody or antigen binding fragment thereof directed against the EDE for use in vaccinating an individual against one or more flaviviruses wherein the EDE is a stabilized recombinant flavivirus are provided. The dimer is: covalently stabilized with at least one disulphide inter-chain bond or one sulfhydryl-reactive crosslinker between the two sE monomers, and/or by being formed as a single polypeptide chain, and/or by linking the two sE monomers through modified sugar, and/or non-covalently stabilized by substituting at least one amino acid residue in the amino acid sequence of at least one sE monomer with at least one bulky side chain amino acid, at the dimer interface or in domain 1 (D1)/domain 3 (D3) linker of each monomer. The dimer is a homodimer or heterodimer of native and/or mutant envelope polypeptides, from DENV-1, DENV-2, DENV-3, DENV-4, Zika and/or other flavivirus.


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