The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2024

Filed:

Mar. 28, 2018
Applicant:

The United States of America, As Represented BY the Secretary, Department of Health and Human Services, Bethesda, MD (US);

Inventors:

Yong-Chen Lu, Rockville, MD (US);

Peter Fitzgerald, Silver Spring, MD (US);

Zhili Zheng, Gaithersburg, MD (US);

Steven A. Rosenberg, Potomac, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/17 (2015.01); C12Q 1/6881 (2018.01); G16B 30/10 (2019.01); G16B 30/20 (2019.01); C07K 14/725 (2006.01); C12N 5/0783 (2010.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6881 (2013.01); A61K 35/17 (2013.01); C07K 14/7051 (2013.01); C12N 5/0636 (2013.01); C12N 15/1003 (2013.01); C12N 15/1096 (2013.01); G16B 30/10 (2019.02); G16B 30/20 (2019.02); C12N 2502/30 (2013.01);
Abstract

Disclosed are methods of isolating paired T cell receptor (TCR) alpha and beta chain sequences, or an antigen-binding portion thereof. Also disclosed are methods of automatically identifying the TCR alpha and beta chain V segment sequences and CDR3 sequences of a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Methods of preparing a population of cells that express paired TCR alpha and beta chain sequences, or an antigen-binding portion thereof, are also disclosed. Isolated pairs of TCR alpha and beta chain sequences and isolated populations of cells prepared by the methods are also disclosed.


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