The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2024

Filed:

Oct. 23, 2018
Applicants:

Kumiai Chemical Industry Co., Ltd., Taito-Ku, JP;

K.i Chemical Industry Co., Ltd., Iwata, JP;

Okayama Prefectural Government, Okayama, JP;

Inventors:

Toshinobu Yamazaki, Taito-Ku, JP;

Shingo Hattori, Iwata, JP;

Tatsuya Horiuchi, Iwata, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 1/04 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C12N 1/04 (2013.01); C12N 2500/34 (2013.01); C12N 2500/60 (2013.01);
Abstract

Provided herein is a culturing method for bacteria of the genuswhereby a low viscosity can be maintained in a culture solution during the culturing of and at the end of culturing of said bacteria, and that enables easy and efficient post-procedures, including concentration and separation of viable bacteria, among others. This is achieved by growingbacteria by liquid culture using a medium containing a disaccharide (for example, maltose, trehalose, lactose, sucrose) accounting for at least 25 mass % of the carbon source in the medium while maintaining a pH of 5.0 to 7.0 in the culture solution throughout the course of the culture, namely from the beginning of the culturing to the end of the culturing of said bacteria.


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